盤尼西林的過敏反應可分為立即反應與延遲反應。盤尼西林皮膚測試僅能偵測第一型過敏反應，但對於第二、三及四型反應，皮膚試驗無法偵測出來。且第一型的過敏反應會隨時間而減少。建議在給予盤尼西林藥物前，應詳細詢問病人盤尼西林過敏史，以幫助臨床判斷。若病人曾有盤尼西林過敏史，建議避免使用盤尼西林；若病人臨床上有必要使用盤尼西林，則考慮實施盤尼西林皮膚測試，測試結果若為陽性，又非得使用盤尼西林，須進行減敏。完整的盤尼西林皮膚測試試劑，必須同時包括主要與次要之決定因子。目前國內醫學中心所採用之盤尼西林皮膚測試試劑多以penicillin G 新鮮配製，此試劑僅包括一個次要決定因子而無主要決定因子。因此，須重新思考實行盤尼西林皮膚測試的對象和方法，及如何執行一個完整的盤尼西林皮膚測試。

Penicillin allergic reactions can be classified into immediate reaction and delayed reaction; and penicillin skin test can only detect Type I allergic reaction, while Types II, III, and IV allergic reactions cannot be detected. However, allergic reactions to immunoglobulin E mediated penicillin will decrease over time, so it is recommended that before administering penicillin to patients, physicians should ask about the allergic history and record it on a chart to help clinical diagnosis. If the patient has a history of penicillin allergy, it is recommended to avoid penicillin. It is considered to perform the penicillin skin test to identify the risk of immediate penicillin reactions if the patient is clinically necessary to use penicillin. If the result is positive, and penicillin is the best choice of treatment for the patient, and then, penicillin must be used and desensitization is required. The complete penicillin skin test reagent should include both major and minor determinants. In Taiwan, the penicillin skin test reagents, currently used by many medical centers, is preparing with fresh penicillin G, and it includes only one minor determinant but without a major determinant. Therefore, it is necessary to comprehensive thinking of the subject and method of penicillin skin test and how to perform penicillin skin test completely.